Background:
@EN It has been reported that ginseng has the antihypertensive effect, immunologic function and promotes anticancer activity. However, there has been no research reports yet about the effect of ginseng on cultured human epidermal cells, and also
its
physiologic concentration is not known.
@ES Objective:
@EN The effects of Korean ginseng (Panax ginseng C.A. Meyer) extracts on the growth of cultured human keratinocytes and melanocytes were investigated.
@ES Methods:
@EN Epidermal Keratinocytes and melanocytes were isolated from the neonatal foreskin and cultured using MCDB 153 and modified TIC media, respectively. Then various concentrations of ginseng extracts(total saponin: TS, panaxadiol saponin: PDS,
panaxatriol saponin : PTS, and water extract:WE) were added to each cultured system. The morphologic changes of epidermal cells were evaluated by a phase-contrast microscope in a 48-hour culture system. Also the effect of ginseng extracts on the
proliferation of epidermal cells and the melanization process of melanocyte were observed in a 6-day in vitro culture system of human keratinocyte and melanocyte.
@ES Results: The results are summarized as follows:
@EN 1. Ginseng saponins (TS, PDS, PTS) and water extract (WE) inhibited proliferation of the cultured keratinocyte in a dose-dependent manner. We observed that ginseng extracts have the antiproliferative effect on the keratinocytes in TS with
concentration of 100㎍/ml or more; PDs, 200㎍/ml or more; PTS, 100㎍/ml or more; and WE, 100㎍/ml or more.
2. Apparent cytotoxic effects on the keratinocyte were observed in the PTS with a concentration of 100㎍/ml or more.
3. There were no effects on the proliferation or melanization process of the melanocyte.
4. Melanocyte became more abundant in cytoplasm, more numerous and shorter in dendrited accoreding to the administered doses of PTS.
@ES Conclusion:
@EN The above results indicated that the addition of ginseng extracts to the tin vitro culture system of human kerationcyte and melanocyte inhibits the proliferation of keratinocytes in a dose-dependent manner, that is, lower concentrations of
ginseng
extracts revealed a cytostatic effect or no growth inhibition on the keratinocyte proliferation; and higher concentrations(100㎍/ml or more) had a cytotoxic effect. These data suggest that Korean ginseng saponins and water extract may play a role
in the
treatment of various skin diseases. And for the clinical application of ginseng, the precise action mechanism of ginseng must be verified by many other experiments. (Kor J Dermatol 1994 ; 32(3) : 451~461)
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